CURRICULUM VITAE

CURRICULUM VITAE

Date: February 1, 2008

Name: Stefan Ambs

Laboratory Address: Laboratory of Human Carcinogenesis

NIH, NCI, CCR

Building 37, Room 3050B

Bethesda, MD 20892-4258

Tel. (301) 496-4668

Fax. (301) 496-0497

Email: ambss@mail.nih.gov

Education:

1980- Graduated from High School, Gymnasium Waldkirch; Waldkirch, Germany

1988- M.S., Biochemistry, University of Tübingen; Tübingen, Germany

1992- Ph.D., Summa cum laude, Pharmacology and Toxicology, Institute of Toxicology,

University of Würzburg; Würzburg, Germany

2005- M.P.H., Epidemiology, Johns Hopkins University; Baltimore, Maryland

Brief Chronology of Employment:

1988 - 1992 Assistant Researcher, Department of Toxicology, University of Würzburg, Würzburg, Germany

1992 - 1997 Visiting Fellow, Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD

1997 - 1998 Research Scientist, Megabios Corporation, Burlingame, CA

1998 - 2001 Senior Research Scientist, Cambridge Genomics Center, Ariad/Aventis Pharmaceuticals, Cambridge, MA

2001 - pres. Tenure-track Investigator, Laboratory of Human Carcinogenesis, National

Cancer Institute, National Institute of Health, Bethesda, MD

Military Service:

1980-1982 Civil Service; Lahr, Germany

Honors, Distinguished Lectures and Other Special Scientific Recognition:

1. Sonderforschungsbereich Predoctoral Fellowship Award, 1988-1992

2. Fogarty Fellowship Award, National Institute of Health, Bethesda, MD, 1992-1997

3. Fellowship Award, Deutsche Forschungsgemeinschaft (DFG), 1993-1994

4. Presenter, 4^th International Meeting on the Biology of Nitric Oxide, Amelia Island,

FL, 1996

5. Presenter, 4^th International Meeting of the Oxygen Society, San Francisco, CA, 1997

6. Participant, NCI State of Science Meeting in Lung Cancer, Bethesda, MD, 1999

7. Co-organizer, “Molecular Carcinogenesis, Chemoprevention, Epidemiology and

Diagnosis of Lung Cancer” Conference, Bethesda, MD, 2003

8. Presenter, NCI Workshop “Free Radicals, Oxidative Stress and Inflammation in

Cancer”, Bethesda, MD, 2007

9. Co-organizer, NCI Workshop “Profiling of Immune Response to Guide Cancer

Diagnosis, Prognosis, and Prediction of Therapy”, Bethesda, MD, 2007

Editorial Boards

1. Carcinogenesis (2008 - pres)

Reviewer (Ad hoc) for the following Peer-reviewed Journals:

American Journal of Pathology

BMC Cancer

Cancer

Cancer Research

Carcinogenesis

Cancer Epidemiology, Biomarker & Prevention

International Journal of Cancer

Journal Clinical Oncology

Journal of the National Cancer Institute

PNAS

Molecular Carcinogenesis

Societies:

American Association for Cancer Research

American Association of Advancement of Science

Research Interests:

Molecular epidemiology and cancer risk assessment; mechanisms of carcinogenesis; inflammation and angiogenesis; nitric oxide biology.

1. Sabbioni, G., Ambs, S., Wogan, G. N. and Groopman, J. D.: The aflatoxin-lysine adduct quantified by high performance liquid chromatography from human serum albumin samples. Carcinogenesis, 11: 2063-2066, 1990.

2. Felley‑Bosco, E., Ambs, S., Lowenstein, C. J., Keefer, L. K. and Harris, C. C.: Constitutive expression of inducible nitric oxide synthase in human bronchial epithelial cells induces c‑fos and stimulates the cGMP pathway. Am J Respir Cell Mol Biol., 11: 159‑164, 1994.

3. Neumann, H. G., Ambs, S. and Bitsch, A.: The role of nongenotoxic mechanisms in arylamine carcinogenesis. Environ Health Perspect., 6: 173‑176, 1994.

4. Felley‑Bosco, E., Mirkovitch, J., Ambs, S., Mace, K., Pfeifer, A., Keefer, L. K. and Harris, C. C.: Nitric oxide and ethylnitrosourea: relative mutagenicity in the p53 tumor suppressor and hypoxanthine‑phosphoribosyltransferase genes. Carcinogenesis, 16: 2069‑2074, 1995.

5. Tas, P., Ambs, S., Stopper, H., Koschel, K., and Schiffmann, D.: Role of extracellular calcium and calcium stores in the intracellular calcium rise induced by diethylstilboestrol in C6 rat glioma cells. Toxicol. in Vitro, 9, 117-121, 1995.

6. Forrester, K., Ambs, S., Lupold, S. E., Kapust, R. B., Spillare, E. A., Weinberg, W. C., Felley‑Bosco, E., Wang, X. W., Geller, D. A., Tzeng, E., Billiar, T. R. and Harris, C. C.: Nitric oxide‑induced p53 accumulation and regulation of inducible nitric oxide synthase expression by wild‑type p53. Proc Natl Acad Sci USA, 93: 2442‑2447, 1996.

7. Ambs, S., and Neumann, H.-G.: Acute and chronic toxicity of aromatic amines studied in the isolated perfused rat liver. Toxicol. Applied Pharmacol., 139: 186-194, 1996.

8. Ambs, S., Hussain, S. P. and Harris, C. C.: Interactive effects of nitric oxide and the p53 tumor suppressor gene in carcinogenesis and tumor progression. FASEB J., 11: 443-448, 1997.

9. Kane, J. M., Shears, L. L., Hierholzer, C., Ambs, S., Billiar, T. and Posner, M. C.: Chronic hepatitis C virus infection in humans - induction of hepatic nitric oxide synthase and proposed mechanisms for carcinogenesis. J. Surg. Res., 69: 321-324, 1997.

10. Ambs, S., Merriam, W. G., Bennett, W. P., Ogunfusika, M. O., Oser, S. M., Klein, S., Felley-Bosco, E., Billiar, T. R. and Harris, C. C.: Frequent nitric oxide synthase-2 expression in human colon adenomas: Implication for tumor angiogenesis colon cancer progression. Cancer Res., 58: 334-341, 1998.

11. Ambs, S., Bennett, W. P., Merriam, W. G., Ogunfusika, M. O., Oser, S. M., Khan, M. A,

Jones, R. T. and Harris, C. C.: Vascular endothelial growth factor and nitric oxide synthase expression in human lung cancer and the relation to p53. Br. J. Cancer, 78: 233-239, 1998.

12. Ambs, S., Ogunfusika, M. O., Merriam, W. G., Bennett, W. P., Billiar, T. R. and Harris, C.C.: Upregulation of NOS2 expression in cancer-prone p53 knockout mice. Proc. Natl. Acad. Sci USA, 95: 8823-8828, 1998.

13. Ambs, S., Merriam, W. G., Ogunfusika, M. O., Bennett, W. P., Ishibe, N., Tzeng, E., Geller, D. A., Billiar, T. R. and Harris, C. C.: p53 and vascular endothelial growth factor regulate tumour growth of NOS2-expressing human carcinoma cells. Nature Med., 4: 1371-1376, 1998.

14. Ambs, S., Bennett, W. P., Merriam, W. G., Ogunfusika, M. O., Oser, S. M., Harrington, A. M., Shields, P. G., Felley-Bosco, E., Hussain, S. P. and Harris, C. C.: Characteristic p53 mutations correlate with inducible nitric oxide synthase expression in human colorectal cancer. J. Natl. Cancer Inst., 91: 86-88, 1999.

15. Ambs, S. and Harris, C. C.: Response: Re: Relationship between p53 mutations and inducible nitric oxide synthase expression in human colorectal cancer. J. Natl. Cancer Inst., 91: 1510-1511, 1999.

16. Ambs, S., Dennis, S., Fairman, J., Wright, M. and Papkoff, J.: Inhibition of tumor growth correlates with the expression level of a human angiostatin transgene in transfected B16F10 melanoma cells. Cancer Res., 59: 5773-5777, 1999.

17. Ambs, S., Hussain, S. P., Marrogi, A. J. and Harris, C. C.: Cancer-prone oxyradical overload disease. IARC Sci. Publ., 150: 295-302, 1999.

18. Hussain, S. P., Amstad, P., Raja, K., Ambs, S., Nagashima, M., Bennett, W. P., Shields, P. G., Ham, A. J., Swenberg, J. A., Marogi, A. J. and Harris, C. C.: Increased p53 mutation load in noncancerous colon tissue from ulcerative colitis: a cancer-prone chronic inflammatory disease. Cancer Res., 60: 3333-3337, 2000.

19. Goodman, J.E., Mechanic, L.E., Luke, B.T., Ambs, S., Chanock, S., and Harris, C.C.: Exploring SNP-SNP interactions and colon cancer risk using polymorphism interaction analysis. Int. J. Cancer, 118: 1790-1797, 2006.

21. Boersma, B.J., Howe, T.M., Goodman, J.E., Yfantis, H.G., Lee, D.H., Chanock, S.J., and Ambs, S.: Association of breast cancer outcome with status of p53 and MDM2 SNP309. J. Natl. Cancer Inst., 98: 911-919, 2006.

22. Thomas, D.D., Ridnour, L.A., Espey, M.G., Donzelli, S., Ambs, S., Hussain, S.P., Harris, C.C., DeGraff, W., Roberts, D.D., Mitchell, J.B., and Wink, D.A.: Superoxide fluxes limit nitric oxide-induced signaling. J. Biol. Chemistry, 281: 25984-25993, 2006.

23. Martin, D.A., Boersma, B.J., Howe, T.M., Goodman, J.E., Mechanic, L.E., Chanock, S.J., and Ambs, S. Association of MTHFR gene polymorphisms with breast cancer survival. BMC Cancer, 6: 257, 2006.

24. Prueitt, R.L., Boersma, B.J., Howe, T.M., Goodman, J.E., Thomas, D.D., Ying, L., Pfiester, C.M., Yfantis, H.G., Cottrell, J.R., Lee, D.H., Remaley, A.L., Hofseth, L.J., Wink, D.A., and Ambs, S. Inflammation and IGF-I activate the Akt pathway in breast cancer. Int. J. Cancer, 120: 796-805, 2006.

25. Bernig, T., Boersma, B.J., Howe, T.M., Welch, R., Yadavalli, S., Staats, B., Mechanic, L.E., Chanock, S.J., and Ambs, S. The Mannose-binding lectin (MBL2) haplotype and breast cancer: an association study in African American and Caucasian women. Carcinogenesis, 28: 828-836, 2007.

26. Pine, S.R., Mechanic, L.E., Ambs, S., Bowman, E.D., Chanock, S.J., Loffredo, C., Shields, P.G., and Harris, C.C. Association of lung cancer outcome with functional polymorphisms in mannose-binding lectin 2, an innate immunity gene. JNCI, 99: 1401-1409, 2007.

27. Crawford, N.P.S., Qian, X., Ziogas, A., Papageorge, A.G., Boersma, B.J., Walker, R.C., Lukes, L., Rowe, W.H., Zhang, J., Ambs, S., Lowy, D.R., Anton-Culver, H., and Hunter, K.W. Rrp1b, a new candidate susceptibility gene for breast cancer progression and metastasis. PLOS Genetics, 3: e214, 2007.

28. Boersma, B.J., Reimers, M., Yi, M., Ludwig, J.A., Luke, B.T., Stephens, R.M., Yfantis, H.G., Lee, D.H., Weinstein, J.N., and Ambs, S. A stromal gene signature associated with inflammatory breast cancer. Int. J. Cancer, 122: 1324-1332, 2008.

29. Wallace, T.A., Prueitt, R.L., Yi, M., Howe, T.M., Gillespie, J.W., Yfantis, H.G., Stephens, R.M., Caporaso, N.E., Loffredo, C.A., and Ambs, S. Tumor immunobiological differences in prostate cancer between African-American and European-American men. Cancer Res., 68: 927-936, 2008.

30. Ambs, S., Prueitt, R.L., Yi, M., Hudson, R.S., Howe, T.M., Petrocca, F., Wallace, T.A., Liu, C.-G., Volinia S., Calin, G.A., Yfantis, H.G., Stephens, R.M., and Croce, C.M. Genomic profiling of microRNA and mRNA reveals deregulated microRNA expression in prostate cancer. Cancer Research., submitted.

31. Prueitt, R.L., Yi, M., Hudson, R.S., Wallace, T.A., Howe, T.M., Yfantis, H.G., Stephens, R.M., Liu, C.-G., Calin, G.A., Croce, C.M., and Ambs, S. MicroRNA and mRNA expression profiles associated with perineural invasion in prostate cancer. The Prostate, submitted.

Chapters in Books

1. Neumann, H. G., Ambs, S. and Hillesheim, W.: The biochemical basis of hepatotoxicity. In Dekant, W. and Neumann, H. G. (Eds.): Tissue-Specific Toxicity: Biochemical Mechanisms. Academic Press, London, 1992, pp. 139-162.

2. Ambs, S., Forrester, K., Lupold, S. E., Merriam, W. G., Geller, D. A., Billiar, T. R. and Harris, C. C.: Regulation of human inducible NOS2 by the tumor suppressor gene p53. In Moncada, S., Stamler, J., Gross, S. and Higgs, E. A. (Eds.): The Biology of Nitric Oxide, Part 5. Portland Press, London, 1996, pp. 7.

Patent applications

1. Ambs, S. and Harris, C.C. “p53 and VEGF regulate tumor growth of NOS2 expressing cancer cells”. Patent Application No. E-223-1998/0-US-04; 358200US

2. Boersma, B.J., and Ambs, S. “Manganese superoxide dismutase Val16Ala polymorphism predicts resistance to doxorubicin cancer therapy”. Patent Application No. PCT/US2007/068588